The Museo Nacional Ruta del Esclavo (Slave Route National Museum) is housed in San Severino Castle, in the Matanzas district of Cuba. It opened in 2009, as the product of UNESCO's Slave Route project. Off the beaten tourist track, the museum in the seventeenth-century castle receives few visitors.

The museum itself houses an important exhibition, aimed at showcasing the horrors of the Transatlantic Slave Trade in order to prevent the like ever happening again. The museum has four exhibition rooms with photographs, objects and archaeological items accompanied by interpretive text. These rooms address the origins of slavery, the plantation systems, abolition and emancipation, and the ongoing legacies affecting people of African heritage around the world.

After being selected by UNESCO to house interpretation for the Slave Route project, the museum has supported a number of African cultural groups in the local area, as well as hosting activities for the nearby university. In the exhibition space itself, there are fourteen African sculptures donated by Cuban artist Lorenzo Padilla.